Historical dating

Since 1995, at least 100 plant biogeographic studies have used molecular-clock dating, and about a fifth has used relaxed clocks.Many of these report evidence of long-distance dispersal.Meta-analyses of studies from the same geographic region can identify directional biases because of prevailing wind or water currents and the relative position and size of landmasses.

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However, we were able to successfully determine tree age and develop a ring-width chronology using standard dendrochronological techniques.Age estimation from molecular sequences has emerged as a powerful tool for inferring when a plant lineage arrived in a particular area.Knowing the tenure of lineages within a region is key to understanding the evolution of traits, the evolution of biotic interactions, and the evolution of floras.We then deal with the question of optimizing the analytical process, especially in view of the most suitable grain size.In the focus of our investigations we have tested several methods of evaluating frequency distributions based on single aliquot/multiple grain measurements.

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